生态无极 发表于 2010-6-13 20:56:27

[原创]

 
<p style="LINE-HEIGHT: 19.5pt"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">麦粒培养基</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></p>
<p style="LINE-HEIGHT: 19.5pt"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">麦粒</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> 80</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">木屑</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> 18</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">蔗糖</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> 1</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">石膏粉</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> 1</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <o:p></o:p></span></p>
<p style="LINE-HEIGHT: 19.5pt"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">制法是先将麦粒洗净,浸泡一夜吸水膨胀后,置锅中煮沸半小时,至熟而不烂,捞起滤去多余的水,加入其它配料混拌均匀。调节好适宜的酸碱度,含水量为</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">60</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%~</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">65</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%,然后逐一分装菌种瓶中,边装边压实,装至瓶高</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">1/2</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">或</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">3/5</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">处压平(应在表面铺一层木屑后压平),中间扎一圆洞,洗净瓶壁和瓶口,用布擦干,塞上棉花,并用牛皮纸包扎好瓶口。</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <o:p></o:p></span></p>

生态无极 发表于 2010-6-13 20:57:19

[原创]

&nbsp;
<p><font size="4"><font color="#0000ff"><b>嵌入式菌种制作方法,将原料按配方加水搅拌均匀(以上两种配方均可),至含水量</b><b>65%</b><b>,将培养料从多功能嵌入式种模两边的孔装入,</b><b>15-20</b><b>支种模装入塑料袋中,缝隙中加满培养料,套环封口,进行高温灭菌,灭菌结束,冷却至</b><b>25</b><b>℃时在无菌接入菌种,放入培养室进行培养,发满后既可用于生产袋接种。</b></font></font><b></b></p>

生态无极 发表于 2010-6-13 20:59:27

[原创]

<font color="#0000ff" size="4">&nbsp; </font>
<p style="LINE-HEIGHT: 19.5pt"><font size="4"><font color="#0000ff"><font color="#0000ff"><font color="#0000ff"><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">2.</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">培养基灭菌</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span></font><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma"><font color="#0000ff">培养基配制并装好好后</font><font color="#0000ff">,</font><font color="#0000ff">即可进行高压灭菌。</font></span></font><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></font></font></p>
<p style="LINE-HEIGHT: 19.5pt"><font size="4"><font color="#0000ff"><font color="#0000ff"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma"><font color="#0000ff">高压灭菌</font></span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span></font><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma"><font color="#0000ff">利用高压锅产生的蒸汽压进行杀菌。具体操作,把料瓶装火锅内</font>,</span><span style="FONT-SIZE: 15pt">进行加热,当压力达到<span lang="EN-US">0.04—0.05</span>兆帕时,打开放气阀,使锅内蒸汽连同冷空气一同排出,此时放气阀排出的蒸汽成直线,直线长度<span lang="EN-US">5-10</span>公分,待锅内气体排净后,压力表归零,再关闭放气阀,继续加热至<span lang="EN-US">0.147</span>兆帕,维持<span lang="EN-US">120-180</span>分钟。灭菌结束,停止加热,自然冷却,当压力表归零后,打开排气阀,然后将锅盖打开一小缝使热蒸<font color="#0000ff">汽溢出,当培养基降至<span lang="EN-US">60</span>℃左右时</font></span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma"><font color="#0000ff">取出料瓶<font size="3">。</font></font></span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span></font></font></p>

生态无极 发表于 2010-6-13 21:00:22

[原创]

<font color="#0000ff">&nbsp; </font>
<p style="LINE-HEIGHT: 19.5pt"><font color="#0000ff"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">(二)接种、培养</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></font></p>
<p style="LINE-HEIGHT: 19.5pt"><font color="#0000ff"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">培养基经配制和灭菌后,便可进行接种。为避免接种过程中污染杂菌,接种工作要在无菌室或接种箱中进行。室内装有紫外线杀菌灯,门窗关闭后里面的空气能与外界隔绝,经消毒就能造成无菌条件。接种箱是代替无菌室的密闭透明小箱。具有体积小,简单轻便,消毒彻底等优点。一般长</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">100</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">厘米,宽</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">60</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">厘米,高</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">50</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">~</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">60</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">厘米,顶部向两边倾斜,安装透明玻璃。箱里装有紫外线杀菌灯,或用福尔马林和高锰酸钾混合熏蒸灭菌,人在箱外,只用两只手伸入箱内进行接种操作,效果好,成功率高。</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <o:p></o:p></span></font></p>

生态无极 发表于 2010-6-13 21:01:16

[原创]

&nbsp;
<p style="LINE-HEIGHT: 19.5pt"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">培养基经灭菌之后,在无菌室或接种箱中,用接种针在酒精灯火焰上,迅速从试管母种中挑一小块带有培养基的菌丝体,放在瓶内的培养基上即成原种。接种后的原种应放在培养室中培养,温度保持在</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">23</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">~</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">26</span><span style="FONT-SIZE: 15pt; COLOR: #333333">℃</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">之间,待菌丝长满全瓶后,即可用来接栽培种或栽培袋。</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></p>

生态无极 发表于 2010-6-13 21:01:44

[原创]

<span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-bidi-font-family: Tahoma; mso-font-kerning: 1.0pt; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">栽培种的培养基其配制方法和灭菌等大致与原种相同,但装瓶应满些,至瓶肩处为宜。一般每瓶原种可转接栽培种</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma; mso-fareast-font-family: 宋体; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">60</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-bidi-font-family: Tahoma; mso-font-kerning: 1.0pt; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">~</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma; mso-fareast-font-family: 宋体; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">80</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-bidi-font-family: Tahoma; mso-font-kerning: 1.0pt; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">瓶。</span>

生态无极 发表于 2010-6-13 21:02:10

[原创]

&nbsp;
<p style="LINE-HEIGHT: 19.5pt"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">菌种的鉴定和保存</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></p>
<p style="LINE-HEIGHT: 19.5pt"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">(一)菌种的鉴定</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <o:p></o:p></span></p>
<p style="LINE-HEIGHT: 19.5pt"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">菌种的质量,对食用菌的产量和质量影响极大。因此,在培育菌种时,要认真对待,严格把关,选用优良菌株,逐级扩大。在投入生产之前,必须进行菌种质量的鉴定,并通过小面积的栽培试验,证实是优良菌种后,再推广使用。常用鉴别菌种质量的方法,有以下几种:</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <span lang="EN-US"><o:p></o:p></span></span></p>

生态无极 发表于 2010-6-13 21:02:41

[原创]

&nbsp;
<p style="LINE-HEIGHT: 19.5pt"><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">1.</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">感官鉴别法</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">主要是通过肉眼观察菌丝的生长情况和用鼻闻菌种的气味等方式来鉴别菌种。一般优良的菌种应具有种性纯,菌丝健壮,分枝浓密,生长整齐,无杂菌或老化现象,同时各具有该食用菌的香气。</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></p>

生态无极 发表于 2010-6-13 20:53:07

[原创]

<font color="#0000ff" size="4">&nbsp; </font>
<p style="LINE-HEIGHT: 19.5pt"><font size="4"><font color="#0000ff"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">五、母种分离材料的选择</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></font></font></p>
<p style="LINE-HEIGHT: 19.5pt"><font size="4"><font color="#0000ff"><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">1.</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">子实体的选择</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">无论是用作孢子分离或组织分离的子实体,都要从产量高,长势好,适应性强,无杂菌虫害的群体中,选择朵大,生长健壮的单生子实体作为分离的材料。</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <o:p></o:p></span></font></font></p>
<p style="LINE-HEIGHT: 19.5pt"><font size="4"><font color="#0000ff"><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">2.</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">组织分离法</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">是用食用菌幼嫩的组织块直接分离培养成纯菌种的方法。选用的子实体先经</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">0.1</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%开汞水或</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">70</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">%酒精表面消毒后。用无菌水冲洗并用无菌纱布擦去表面水分。分离时用无菌解剖刀自菌柄处切开少许,再用手将子实体掰开为二,在菌盖与菌褶交界处,切取</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">3</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">~</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">4</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">毫米的一小块菌肉,移放在斜面培养基中央。组织分离后将试管外面放在恒温箱中</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">25</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">——</span><?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" /><st1:chmetcnv w:st="on" unitname="℃" sourcevalue="27" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">27</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">℃</span></st1:chmetcnv><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">培养。待组织块周围萌发出菌丝,并向</span><span style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> </span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">培养基蔓延生长后,再挑取生长健壮的菌丝进行转管培养。移入试管斜面培养基上,经</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">25</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">~</span><st1:chmetcnv w:st="on" unitname="℃" sourcevalue="27" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">27</span><span style="FONT-SIZE: 15pt; COLOR: #333333">℃</span></st1:chmetcnv><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">培养,菌丝生长后,再行转管培养。组织分离法操作简便,又不易带入杂菌,容易获得纯菌种。</span></font></font><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"><o:p></o:p></span></p>

生态无极 发表于 2010-6-13 20:53:53

[原创]

&nbsp;
<p style="LINE-HEIGHT: 19.5pt"><font color="#0000ff"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">六、母种的扩大培养</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"> <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></span></font></p>
<p style="LINE-HEIGHT: 19.5pt"><font color="#0000ff"><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">从分离获得或外地引入的优良母种,由于数量有限不能满足生产上的需要,应进行扩大繁殖培养。其方法是以无菌操作把斜面培养基连同菌丝体切成米粒大的小块,移接到新的斜面培养基上,在适温条件下培养,待菌丝长满斜面时即成。一般每支母种试管可扩大繁殖</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">60</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">~</span><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma">80</span><span style="FONT-SIZE: 15pt; COLOR: #333333; mso-bidi-font-family: Tahoma; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma">支新管。扩大母种第一次应多些,以避免多次转管,造成菌丝生活力降低,结菇少,影响产量和质量。一般要求转管不要超过五次。</span></font><span lang="EN-US" style="FONT-SIZE: 15pt; COLOR: #333333; FONT-FAMILY: Tahoma"><o:p></o:p></span></p>
页: 1 [2] 3 4 5 6 7 8
查看完整版本: [原创]杏鲍菇生产工艺介绍