pla9 发表于 2011-12-7 14:46:20

多糖的纯化

<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-ALIGN: center" align="center"><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">多糖的纯化</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.6</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">处理样品</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><font face="仿宋_GB2312"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US"><span style="mso-spacerun: yes">&nbsp;<wbr>&nbsp;<wbr></span></span></b> <span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">从冰箱内取出样品用研钵研磨成粉,然后称出其质量,记为<span lang="EN-US" XML:LANG="EN-US">m4</span>。</span></font></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.7</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">脂肪的去除</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><font face="仿宋_GB2312"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US"><span style="mso-spacerun: yes">&nbsp;<wbr>&nbsp;<wbr></span></span> <span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">将研磨后的样品溶于石油醚中,充分振荡后离心(<span lang="EN-US" XML:LANG="EN-US">4500</span>转<span lang="EN-US" XML:LANG="EN-US">/min</span>,<span lang="EN-US" XML:LANG="EN-US">4min</span>)取其沉淀。</span></font></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.8</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">多糖、色素和蛋白的去除</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><font face="仿宋_GB2312"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US"><span style="mso-spacerun: yes">&nbsp;<wbr></span><span style="mso-spacerun: yes">&nbsp;<wbr></span><span style="mso-spacerun: yes">&nbsp;<wbr></span></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">将沉淀溶于<span lang="EN-US" XML:LANG="EN-US">50</span>摄氏度<span lang="EN-US" XML:LANG="EN-US">80%</span>酒精<span lang="EN-US" XML:LANG="EN-US">50ml</span>中并用玻璃棒搅拌,充分搅拌后再次离心(<span lang="EN-US" XML:LANG="EN-US">4500</span>转<span lang="EN-US" XML:LANG="EN-US">/min</span>,<span lang="EN-US" XML:LANG="EN-US">4min</span>)取其沉淀。</span></font></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.9</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">热水浸提</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><font face="仿宋_GB2312"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US"><span style="mso-spacerun: yes">&nbsp;<wbr></span> <span style="mso-spacerun: yes">&nbsp;<wbr></span></span></b><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">离心后的沉淀放在<span lang="EN-US" XML:LANG="EN-US">50</span>摄氏度的水浴中进行浸提。<span lang="EN-US" XML:LANG="EN-US">1.5h</span>后离心(<span lang="EN-US" XML:LANG="EN-US">4500</span>转<span lang="EN-US" XML:LANG="EN-US">/min</span>,<span lang="EN-US" XML:LANG="EN-US">4min</span>)取其沉淀。</span></font></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.10</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">有机溶剂沉淀</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><font face="仿宋_GB2312"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US"><span style="mso-spacerun: yes">&nbsp;<wbr>&nbsp;<wbr></span></span></b> <span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">把再次离心后的多糖沉淀物溶于用<span lang="EN-US" XML:LANG="EN-US">95%</span>的酒精中,再次离心(<span lang="EN-US" XML:LANG="EN-US">4500</span>转<span lang="EN-US" XML:LANG="EN-US">/min</span>,<span lang="EN-US" XML:LANG="EN-US">4min</span>)取其沉淀。</span></font></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.11</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">保存</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><font face="仿宋_GB2312"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US"><span style="mso-spacerun: yes">&nbsp;<wbr>&nbsp;<wbr></span></span> <span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">称出其质量记为<span lang="EN-US" XML:LANG="EN-US">m5</span>后,放入<span lang="EN-US" XML:LANG="EN-US">-20</span>摄氏度冰箱内保存。</span></font></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-ALIGN: center" align="center"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312">制备茶多酚的实验设计</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">1</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">样品的处理:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">1</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)称量:将新鲜的香菇洗净后用托盘天平称量其质量;</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2)</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">磷酸缓冲液的配置:分别配制<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">0.2mol/l</span>的磷酸二氢钠<span lang="EN-US" XML:LANG="EN-US">147ml</span>和磷酸氢二钠<span lang="EN-US" XML:LANG="EN-US">53ml</span>,然后加热混合起来并摇匀。</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)切碎:称量后的香菇用刀再案板上尽量切碎;</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">3</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)剪碎:用剪刀将切碎的样品剪碎;</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">4</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)研磨:切碎后的香菇再放入研钵中并加入磷酸缓冲液研磨。</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2</span><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">离心</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">将香菇的研磨液离心<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">4000</span>转每分,<span lang="EN-US" XML:LANG="EN-US">10</span>分钟。</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">3</span><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">再离心</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">倒出并保留离心后的上清液,再分别用磷酸缓冲液和少量蒸馏水离心<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">4000</span>转每分,<span lang="EN-US" XML:LANG="EN-US">10</span>分钟。</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">4</span><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">分别萃取</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">将上清液分为两部分,一部分加无水硫酸钠<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">20g</span>另一部分加活性炭进行萃取。</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5</span><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">保存</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span style="FONT-SIZE: 12pt; COLOR: black; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">静置片刻后离心<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">4000</span>转每分、<span lang="EN-US" XML:LANG="EN-US">10</span>分钟,并称取质量。</font></span> </p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt">&nbsp;</p>

pla9 发表于 2011-12-7 16:50:24

<p>TEST,</p>
<p>TEST,</p>
<p>TEST,</p>
<p>TEST,</p>
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