pla9 发表于 2011-12-7 14:46:04

提取香菇多糖测的实验设计

<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-ALIGN: center" align="center"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312">提取香菇多糖测的实验设计</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">1.</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">样品的处理</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">1</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)称量:将新鲜的香菇洗净后用托盘天平称量其质量,记为<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">m1</span>;</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)切碎:称量后的香菇用刀再案板上尽量切碎;</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">3</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)研磨:切碎后的香菇再放入研钵中研磨,</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">、香菇汁液的提取</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">1</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)加水预热:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">用量筒量取体积为五倍于香菇质量数的蒸馏水,记体积数为<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">v1,</span>然后倒入铝锅中。随后打开电炉给锅内蒸馏水加热至沸腾;</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)装锅:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">再把研磨后的香菇放进煮沸的蒸馏水中,边煮边搅拌以防香菇粘在锅底。当水分减少时,在适当时候往锅内加适量的蒸馏水,定容至<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">v1</span>;</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">3</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)出锅:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">大约煮<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">1.5h</span>后关掉电路的开关,冷却后将香菇汁液倒出,边倒出边进行过滤;</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">3</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">、过滤</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">漏斗下放置一烧杯并使漏斗管贴着烧杯内壁,接着用四层纱布过滤,过滤时尽量把纱布内的和浸到纱布里的汁液过滤到烧杯中,记下过滤后的体积数为<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">v2</span>;</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">4</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">、蒸发浓缩</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">打开电路的开关,使盛有过滤液的烧杯隔着石棉网在电炉上加热蒸发浓缩,直至体积数为<span lang="EN-US" XML:LANG="EN-US"><font face="仿宋_GB2312">=v3</font></span></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-ALIGN: center" align="center"><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">分组实验</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">一组(薛金、王珏、刘秋霞、提晓东、索鹏飞、杨朝军)</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">二组(孟祥兰、孙磊、杨顺、史文文、孟雅娟、李凯娜):</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">三组(秦加乐、李川、陈美玲、杨莎莎、李瑞娟):</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">、多糖的提取与纯化</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-ALIGN: center" align="center"><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">多糖的提取</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.1</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">沉淀</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">1</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)酒精的配置:一组、二组、三组分别配置<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">75%</span>、<span lang="EN-US" XML:LANG="EN-US">85%</span>、<span lang="EN-US" XML:LANG="EN-US">95%</span>浓度的酒精,各组酒精的量与<span lang="EN-US" XML:LANG="EN-US">v3</span>成<span lang="EN-US" XML:LANG="EN-US">1</span>:<span lang="EN-US" XML:LANG="EN-US">5</span>的比例,记体积为<span lang="EN-US" XML:LANG="EN-US">v4</span>。盛装酒精的烧杯并用封口膜密封以免酒精挥发;</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)静止沉淀:各组将各自配置的酒精与浓缩液混合后静置<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">24h</span>,每隔一定时间观察混合液的变化并用相机拍下来;</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.2</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">离心</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">1</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)多糖的分离:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">静止后的三瓶多糖混合液分别把上清液倒入同一烧杯中,剩下用不同浓度酒精沉淀的絮状物装入不同的烧杯里;</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">2</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)装瓶:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">拿一只离心用的瓶子装入絮状物和少许上清液总体积约占瓶子的<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">2/3</span>,并用电子天平称出其质量再拿另一只瓶子装入同等质量的上清液。就这样每两个一组称、装,边称装边及时盖紧瓶盖并在瓶的一侧贴上标签记上组数和酒精浓度。</font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">3</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)离心:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">合上离心机的开关并打开离心机放入盛有需要离心的瓶子。放入时,一组中的两只瓶子(质量相同的一只絮状物、一只上清液的两只瓶子)的坐位要对称。合上离心机后,设置档次<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">n</span>、转速<span lang="EN-US" XML:LANG="EN-US">v</span>和时间<span lang="EN-US" XML:LANG="EN-US">t;</span></font></span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">4</span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">)分离:</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">将离心后瓶子内的液体倒入废液回收处,再将瓶内底部沉淀物用玻璃棒置入培养皿中(不同组瓶内的沉淀物置于不同培养皿中),并用记号笔标明组数和酒精浓度;</span></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><b style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体" XML:LANG="EN-US">5.3</span></b><b style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">烘干</span></b></p>
<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt; TEXT-INDENT: 18pt; mso-char-indent-count: 1.5"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312; mso-hansi-font-family: 宋体">将标记好的培养皿放入烘箱内烘干(<font face="仿宋_GB2312"><span lang="EN-US" XML:LANG="EN-US">121</span>℃,<span lang="EN-US" XML:LANG="EN-US">30min</span>)<span lang="EN-US" XML:LANG="EN-US">,</span>烘干后的各组多糖用培养皿称出其质量并记下数据分别为<span lang="EN-US" XML:LANG="EN-US">m1</span>、<span lang="EN-US" XML:LANG="EN-US">m2</span>、<span lang="EN-US" XML:LANG="EN-US">m3</span>,盖好皿盖避免多糖吸水。</font></span></p>
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<p style="MARGIN: 0cm="0cm"0cm="0cm"0pt"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 仿宋_GB2312" XML:LANG="EN-US">&nbsp;</span></p>
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