事情哪有绝对的?就算培养基完全无菌,那接种过程也要做到完全无菌?
太绝对了,只能是画蛇添足,不要忘了食用菌原本可是野生的。 为了个广告如此上贴,历时之久,恒心之治,佩服佩服。<br/><br/> 要不这么说吧:<br/> “今年过年不种菇,种菇就用杂菌清”<br/><br/> 不许反驳! <div class="msgheader">QUOTE:</div><div class="msgborder"><b>以下是引用<i>sdaaaw123</i>在2009-4-11 9:06:35的发言:</b><br/>你不能一棍子打死,木屑的树种不同,栽培环境不同,木屑的营养差异可是太大啦。大千世界,高含氮的树种有的是,高营养的木屑也有的是。 </div><p><font face="Verdana" color="#61b713"><b>sdaaaw123</b></font>兄,我不是说黑木耳的配方。</p> <div class="msgheader">QUOTE:</div><div class="msgborder"><b>以下是引用<i>云天</i>在2009-4-14 15:53:58的发言:</b><br/><strong><font size="2">[求助]请各位给个建议</font></strong> <span class="font10" style="LINE-HEIGHT: 20px"><img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://www.mbbs.com.cn/skins/Default/topicface/face1.gif" onload="imgresize(this);"/><font color="#afafaf" size="2"> Post By:2009-4-14 15:49:32 </font></span><br/><br/>
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<div id="textstyle_1" style="FONT-SIZE: 11pt; OVERFLOW: hidden; WORD-BREAK: break-all; TEXT-INDENT: 24px; WORD-WRAP: break-word"><font color="#afafaf" size="2"><img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a16.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313de04b88a5f73c5da918f2e98beed8cf75fba6386f51947e920b725a893bebf46e26895c28474d507a9016ac0a0c5f8b7b03c8d15fcffe3eb74f442e79cffa8e5dd5d3ea0" onload="imgresize(this);"/></font> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a17.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313e6db75f1fe6c0f7a6b63a490b91364b0b9f7a978a33973a7ec1d433793c5f3421471ae7254ec5d1322140530277d3d7fc7b2cd2adb9d061a47fb5ba62e626921cf9046d8" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a17.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a32931398570a0c810b29b64836af2f59b0f35b2c1ca54aec54e496679611df948355ecb4452b062a8844bb1b144a080ec81d4cd575eb2573008f0316c3eefcd8079f4525844c78" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a17.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313918cdb13dda32d4c6a2e31ba2e299b62a87fac73b051e409b4f2c230a3feaba994d00cc466061ebae103918ff0eb00b7186352f8b6062415b94c28a427d1a862cfbed6f5" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a16.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a32931336f528360c8e531cbffb29b594acba3b36ae8b18415667e90d6866cbe656b4689a94fd39b174527b43acd258d571c7667552f4283a6a8ebd368ab89750d238afda558001" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a16.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313c760c75ac748a2afbc1e8f0f72d03650d58aa8a573ec1137f3e78f9faaf316105858bd46418e31fd300495f1ff93827dd4f1536af3f199972bea197992fb6941959ff4be" onload="imgresize(this);"/><br/><br/>以上是我同时扩大的试管。(新手)<br/>一、请问图4、5的波浪状菌丝是什么问题?(平菇菌种)<br/>二、图1是金针,请问菌丝绒长是否正常?<br/>三、图2(平菇)黄色的</div></td></tr></tbody></table></div>
<p>你的PDA加富培养基加有酵母粉或牛肉膏吧?一般不要用加富培养基,就用PDA吧,如果平菇、金针菇菌丝在PDA上长的不好,可能有两个原因,一是菌种退化。;二是PDA没做好。如果PDA是做好了的,而菌丝长不好,肯定菌种的活力不强,可用陈旧的纯粗杂木屑(不要加麦麸或玉米粉)反复培养,逐渐提高菌丝活力,直到在纯木屑上长势旺盛,菌丝粗壮、尖端分枝清楚,然后取带菌丝的杂木屑在PDA平板上培养,一段时间后取菌落边沿的菌丝转接在斜面上培养。图4、图5的平菇菌丝出现轮纹,菌丝稀疏、灰白,有两种可能:(1)培养基不适宜或培养温度过高;(2)或许根本就不是平菇菌丝;图2平菇出现黄色,也许是菌种长期用加富培养基转管和保藏,菌种老化退化。图三的杏鲍菇菌丝稀疏、灰白,不正常。图1的金针菇气生菌丝多,有两种可能,一是接种块刚萌发,气生菌丝似乎有点多;二是不同品种的气生菌丝生长情况不相同。<br/></p> 感谢楼上<font color="#61b713">田鸿的答复,谢谢</font> <strong><font size="2">[求助]请各位给个建议</font></strong> <span class="font10" style="LINE-HEIGHT: 20px"><img alt="发帖心情" src="http://www.mbbs.com.cn/skins/Default/topicface/face1.gif"/><font color="#afafaf" size="2"> Post By:2009-4-14 15:49:32 </font></span><br/><br/>
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<div id="textstyle_1" style="FONT-SIZE: 11pt; OVERFLOW: hidden; WORD-BREAK: break-all; TEXT-INDENT: 24px; WORD-WRAP: break-word"><font color="#afafaf" size="2"><img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a16.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313de04b88a5f73c5da918f2e98beed8cf75fba6386f51947e920b725a893bebf46e26895c28474d507a9016ac0a0c5f8b7b03c8d15fcffe3eb74f442e79cffa8e5dd5d3ea0" onload="imgresize(this);"/></font> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a17.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313e6db75f1fe6c0f7a6b63a490b91364b0b9f7a978a33973a7ec1d433793c5f3421471ae7254ec5d1322140530277d3d7fc7b2cd2adb9d061a47fb5ba62e626921cf9046d8" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a17.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a32931398570a0c810b29b64836af2f59b0f35b2c1ca54aec54e496679611df948355ecb4452b062a8844bb1b144a080ec81d4cd575eb2573008f0316c3eefcd8079f4525844c78" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a17.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313918cdb13dda32d4c6a2e31ba2e299b62a87fac73b051e409b4f2c230a3feaba994d00cc466061ebae103918ff0eb00b7186352f8b6062415b94c28a427d1a862cfbed6f5" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a16.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a32931336f528360c8e531cbffb29b594acba3b36ae8b18415667e90d6866cbe656b4689a94fd39b174527b43acd258d571c7667552f4283a6a8ebd368ab89750d238afda558001" onload="imgresize(this);"/> <img style="CURSOR: pointer" onclick="javascript:window.open(this.src);" alt="图片点击可在新窗口打开查看" src="http://a16.photo.store.qq.com/http_imgload.cgi?/rurl4_s=787ec17e006932549b2929ba3a329313c760c75ac748a2afbc1e8f0f72d03650d58aa8a573ec1137f3e78f9faaf316105858bd46418e31fd300495f1ff93827dd4f1536af3f199972bea197992fb6941959ff4be" onload="imgresize(this);"/><br/><br/><br/><br/><br/>以上是我同时扩大的试管。(新手)<br/>一、请问图4、5的波浪状菌丝是什么问题?(平菇菌种)<br/>二、图1是金针,请问菌丝绒长是否正常?<br/>三、图2(平菇)黄色的</div></td></tr></tbody></table> 四、图3是杏鲍菇菌丝是否正常?<br/>五、图6是鸡腿,菌丝是否稀薄?<br/>以上是相同的培养基(加富PDA),只有平菇的菌丝有黄色,请各位帮忙分析下,下次做的时候吸取经验。谢谢!