大家帮我看下培养基有错吗
土豆200克 <span style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">葡萄糖</span><span lang="EN-US" style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-fareast-font-family: 宋体"> 20g</span><span style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA"> </span><span lang="EN-US" style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-fareast-font-family: 宋体"><br/></span><span style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">琼脂</span><span lang="EN-US" style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-fareast-font-family: 宋体"> 20g </span><span style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">水</span><span lang="EN-US" style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-fareast-font-family: 宋体"> 1000mL<br/>pH</span><span style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">值</span><span style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-fareast-font-family: 宋体"> </span><span style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: 宋体; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">自然</span><span lang="EN-US" style="FONT-SIZE: 11pt; COLOR: #333333; FONT-FAMILY: Arial; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-fareast-font-family: 宋体"><br style="mso-special-character: line-break"/><br style="mso-special-character: line-break"/>我分离的是平菇，分离三天了到现在未见菌丝</span> <p>可我还没看见呢。发菌的时候是先从子实体边缘发的吗。？？还有我分离的是根丙行吗？？？</p> <p>我想问一个，你保温了吗？？？？？？</p> 你的室温是多少？萌发的表现是，首先看到接种的组织发白，平菇是非常雪白的，我22号试验分离的平菇菌丝已有6、7毫米了。上个图就容易分辨点， 是PDA培养基没有错，如果温度适宜，操作没问题，三天可以看见少量的菌丝。 <p>我的室温有14—15度左右</p> <div class="msgheader">QUOTE:</div><div class="msgborder"><b>以下是引用<i>ghost9106</i>在2009-11-25 21:21:21的发言：</b><br/><p>不知道你是如何操作的，可不可以详细说下。<br/>你放了几天时间没有污染可能是以下的原因：<br/>你所用的消毒药品是什么，消毒后有没有用无菌水再清洗一遍，有可能消毒药品没有清洗干净使菌丝不易生长；<br/>接种工具在火焰灭菌后有没有冷却，温度太高将菌块烫死了或离酒精灯太近将其烧死了。<br/>温度最好放在其生长的适宜温度这样才好推除其它不确定的因素以便分析问题。</p></div>
<p>我用酒精洗过就接着分离了工具也是用酒精</p> <p>你用的平菇，最好的地方在柄与盖的交界处，这样把表面的杂物用酒精擦洗一下，直接撕开用小刀切下一小块接到斜面里就可以了。培养里立起来放，这样不会有太多的水，有了细菌污染也会随水向下扩散，如果长出了菌丝还可以从试管口一端再分离。</p> <p>还有一点我有的葡萄糖是医用的还是能喝的那种。着个有影响吗？？？</p> 平菇组织分离的时候琼脂量稍微多一点还好做，水浸润了组织块影响萌发